Journal: Journal of Nanobiotechnology

Article Title: Mesenchymal stem cells-derived extracellular vesicles protect against oxidative stress-induced xenogeneic biological root injury via adaptive regulation of the PI3K/Akt/NRF2 pathway

doi: 10.1186/s12951-023-02214-5

Figure Lengend Snippet: Human adipose-derived mesenchymal stem cell extracellular vesicles (hASC-EVs) inhibited oxidative damage, promoted antioxidant activity, and alleviated H 2 O 2 -stimulated oxidative reactivity in dental follicle cells (DFCs). The oxidative stress and antioxidant activity of the experimental group in response to H 2 O 2 stimulation were examined after hASC-EV administration. a Representative images showing DCF (green) and reactive oxygen species (ROS) production in each group. Scale bar = 200 μm. b Representative images showing 8-OHdG (green nuclei) immunostaining results and the cytoskeleton (red) in each group. Scale bar = 50 μm. c Representative images showing the MMP of DFCs, detected using JC-1 staining, which was identified by green fluorescence for the monomeric form of JC-1 and red fluorescence for potential-dependent aggregation. Scale bar = 100 μm. d Histograms showing lipid peroxidation (MDA) concentrations in the different groups. e – g Histograms showing quantification of the mean fluorescence intensity (MFI) of DCFH ( e ), 8-OHdG ( f ), and JC-1 ratio ( g ) in DFCs. h – k Histograms showing the FRAP ( h ), GSH-PX ( i ), SOD ( j ), and CAT ( k ) concentrations in different groups (n = 5 for each group). ** P < 0.01, *** P < 0.001, and **** P < 0.001, compared to the control

Article Snippet: The samples and reaction reagents included those from the Lipid Peroxidation MDA Assay Kit (Dojindo) and the ferric ion reducing antioxidant power (FRAP), superoxide dismutase (SOD), and catalase (CAT) assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Techniques: Derivative Assay, Antioxidant Activity Assay, Immunostaining, Staining, Fluorescence, Control

Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Cell viability changes after spheroid formation of colorectal cancer cells in different environments. (A) The SNU-C5 and SNU-C5/5-FUR cells were mock-treated with DMSO or treated with indicated doses of 5-FU for 3 days. The extent of cell viability was determined by MTT assay. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. DMSO; # P<0.05 and ## P<0.01 vs. sphere/GF. (B) The SNU-C5 and SNU-C5/5-FUR cells were mock-treated with DMSO or treated with indicated doses of 5-FU for indicated days. The extent of cell viability was determined by MTT assay using the aforementioned procedures. Data are presented as the mean ± SD (n=3). (C) Expression levels of cell cycle-related proteins in monolayer and spheroid formation cultures in SNU-C5 and SNU-C5/5-FUR cells were detected by immunoblotting. Immunoblotting analysis was performed for p21, β-catenin and GSK3β, while GAPDH was used for a loading control. Band density was analyzed by AzureSpot analysis software, and results are expressed as the mean ± SD (n=3). **P<0.01 and ***P<0.001 vs. monolayer; ## P<0.01 vs. sphere/FBS. 5-FU, 5-fluorouracil; FBS, fetal bovine serum; GF, growth factor.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: MTT Assay, Expressing, Western Blot, Control, Software

Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker